产品详情
产品名称Fibronectin Rabbit mAb
克隆号JF0582
来源种属Recombinant Rabbit
克隆性Monoclonal
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Human;Mouse
免疫原描述recombinant protein
标记Unconjugated
别名CIG antibody Cold insoluble globulin antibody Cold-insoluble globulin antibody DKFZp686F10164 antibody DKFZp686H0342 antibody DKFZp686I1370 antibody DKFZp686O13149 antibody ED B antibody Fibronectin 1 antibody FINC antibody FINC_HUMAN antibody FN antibody FN1 antibody FNZ antibody GFND antibody GFND2 antibody LETS antibody Migration stimulating factor antibody MSF antibody Ugl-Y3 antibody
数据库入口号Swiss-Prot#:P02751
Uniprot
P02751
计算分子量272 kDa
实际分子量272 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Fibronectin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Fibronectin antibody. Counter stained with hematoxylin.
ICC staining Fibronectin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Fibronectin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Fibronectin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes: Fibronectin Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates Lane 2 : Human serum cell lysates Lane 3 : Mouse serum cell lysates Lane 4 : Rat serum cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 272 kDa
Observed band size: 272 kDa
Exposure time: 10 seconds
All lanes:Fibronectin Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 :Fibronectin Rabbit mAb knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease.