产品详情
产品名称beta Actin(HRP conjugated) Rabbit mAb
克隆号JF53-10
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB
种属反应性Hu, Ms, Rt, Monkey, Hamster, Plant, zebrafish
免疫原描述recombinant protein
标记HRP
别名A26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Actx antibody
b actin antibody
Beta cytoskeletal actin antibody
Beta-actin antibody
BRWS1 antibody
E430023M04Rik antibody
MGC128179 antibody
PS1TP5 binding protein 1 antibody
PS1TP5BP1 antibody
数据库入口号Swiss-Prot#:P60709
Uniprot
P60709
计算分子量42 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
Western blot analysis of beta Actin on different lysates using anti-beta Actin antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: PC-12
Lane 3: NIH/3T3
Lane 4: zebrafish
Western blot analysis of beta Actin on hybrid fish (crucian-carp) brain tissue lysate using anti-beta Actin antibody at 1/500 dilution.
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
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et al,Genetic Characterization of Primordial Germ Cells in Spotted Sea Bass (Lateolabrax maculatus)
, (2025),
PMID:
41009958