产品详情
产品名称TDP43 Rabbit mAb
克隆号JM51-10
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, IP, ICC/IF, IHC, FC
种属反应性Human;Mouse;Rat;Zebrafish
免疫原描述recombinant protein
标记Unconjugated
别名ALS10 antibody
OTTHUMP00000002171 antibody
OTTHUMP00000002172 antibody
OTTHUMP00000002173 antibody
TADBP_HUMAN antibody
TAR DNA binding protein 43 antibody
TAR DNA binding protein antibody
TAR DNA-binding protein 43 antibody
TARDBP antibody
TDP 43 antibody
TDP-43 antibody
TDP43 antibody
数据库入口号Swiss-Prot#:Q13148
Uniprot
Q13148
计算分子量45 kDa
实际分子量45 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:100-1:500
IP: 1:10-1:50
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TDP43 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TDP43 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TDP43 antibody. Counter stained with hematoxylin.
ICC staining TDP43 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TDP43 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TDP43 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with TDP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: TDP43 Rabbit mAb at 1/1k dilutionLane 1 : K562 whole cell lysates Lane 2 : HepG2 whole cell lysates Lane 3 : Mouse brain lysates Lane 4 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 45 kDa Observed band size: 45 kDaExposure time: 7 seconds
All lanes:TDP43 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 :TDP43 Rabbit mAb knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
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