产品详情
产品名称Calcineurin A Rabbit mAb
克隆号JA64-11
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化ProA affinity purified
应用WB, FC,IHC, ICC/IF
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名Alpha isoform formerly PPP2B antibody
Calcineurin A alpha antibody
Calcineurin A1 antibody
CalcineurinA antibody
Calmodulin dependent calcineurin A subunit alpha isoform antibody
Calmodulin-dependent calcineurin A subunit alpha isoform antibody
CALN antibody
CALNA 1 antibody
CALNA antibody
CALNA1 antibody
CAM PRP catalytic subunit antibody
CAM-PRP catalytic subunit antibody
CCN 1 antibody
CCN1 antibody
CNA 1 antibody
CNA alpha antibody
CNA antibody
CNA1 antibody
PP2BA_HUMAN antibody
PPP2B antibody
Ppp3ca antibody
Protein phosphatase 2B catalytic subunit antibody
Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
Protein phosphatase 3 catalytic subunit alpha isozyme antibody
Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
数据库入口号Swiss-Prot#:Q08209
Uniprot
Q08209
计算分子量59 kDa
实际分子量59 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
FC: 1:50-1:100
All lanes: Calcineurin A Rabbit mAb at 1/1k dilution
Lane 1 : 293 whole cell lysates Lane 2 : A431 whole cell lysates Lane 3 : Mouse lung lysates Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Exposure time: 5 seconds
All lanes:Calcineurin A Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 :Calcineurin A Rabbit mAb knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon tissue stained for Calcineurin A using 49514 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Calcineurin A antibody (49514) ICC/IF staining of Calcineurin A in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 49514 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2B family comprises subfamily members PP2B-Aα, PP2B-Aβ and PP2B-Aγ. Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2.
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