产品详情
产品名称GCLM Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC, IP
种属反应性Human;Mouse;Rat
免疫原描述Recombinant protein
标记Unconjugated
别名Gamma ECS regulatory subunit antibody
Gamma-ECS regulatory subunit antibody
Gamma-glutamylcysteine synthetase regulatory subunit antibody
GCLM antibody
GCS light chain antibody
GLCLR antibody
Glutamate cysteine ligase regulatory subunit antibody
Glutamate--cysteine ligase modifier subunit antibody
Glutamate--cysteine ligase regulatory subunit antibody
GSC light chain antibody
GSH0_HUMAN antibody
数据库入口号Swiss-Prot#:P48507
Uniprot
P48507
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:100
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of GCLM on different cell lysates using anti-GCLM antibody at 1/500 dilution. Positive control: Lane 1: A431 Lane 2: PC-12 Lane 3: NIH-3T3
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GCLM antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GCLM antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-GCLM antibody. Counter stained with hematoxylin.
ICC staining GCLM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GCLM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GCLM in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with GCLM antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Gamma-glutamylcysteine synthetase (γ-GCS) is the rate limiting enzyme for glutathione (L-gamma-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. γ-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human gamma-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human γ-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of γ-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.
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, (2025),
PMID:
41110354