产品详情
产品名称GLO1 Rabbit mAb
克隆号JU44-11
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB,ICC,IHC,FC
种属反应性Human;Mouse;Rat
免疫原描述Recombinant protein
标记Unconjugated
别名Aldoketomutase antibody
glo1 antibody
GLOD1 antibody
Glx I antibody
GLYI antibody
glyoxalase domain containing 1 antibody
Glyoxalase I antibody
Ketone aldehyde mutase antibody
Ketone-aldehyde mutase antibody
Lactoyl glutathione lyase antibody
Lactoylglutathione lyase antibody
LGUL_HUMAN antibody
Methylglyoxalase antibody
S D lactoylglutathione methylglyoxal lyase antibody
S-D-lactoylglutathione methylglyoxal lyase antibody
数据库入口号Swiss-Prot#:Q04760
Uniprot
Q04760
计算分子量21 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:100-1:500
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis tissue
Lane 2: C2C12
Lane 3: 293
Lane 4: Rat spleen tissue
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLO1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-GLO1 antibody. Counter stained with hematoxylin.
ICC staining GLO1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GLO1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GLO1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with GLO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
The glyoxal pathway plays a role in the detoxification of glucose degradation products (GDP). Glyoxalase I (GLO1), a member of the glyoxalase family, is effective in eliminating GDP. Overexpression or silencing of Glyoxalase I in mice brain suggests an association between Glyoxalase I and anxiety. Glyoxalase I has three isoforms generated from two alleles in the genome which forms two homodimers and one heterodimer, each subunit binding one zinc ion. Research demonstrates that GLO1 gene expression is induced in colon carcinoma. Both an insulin response element (IRE), and a zinc metal response element (MRE) in the promoter region of the GLO1 gene have been identified.
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et al,A lactate capture-sensing platform for facilitating early differential diagnosis of minimal change disease and focal segmental glomerulosclerosis in renal diseases
, (2025),
PMID:
41192155