产品详情
产品名称Nuf2 Rabbit mAb
克隆号JG34-64
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB,FC
种属反应性Human
免疫原描述Recombinant protein within human Nuf2 aa 1-200.
标记Unconjugated
别名Cancer/testis antigen 106 antibody
CDCA 1 antibody
CDCA1 antibody
Cell division cycle associated 1 antibody
Cell division cycle associated protein 1 antibody
Cell division cycle-associated protein 1 antibody
CT106 antibody
hNuf 2 antibody
hNuf2 antibody
hNuf2R antibody
hsNuf2 antibody
Kinetochore protein Nuf2 antibody
Nuf 2 antibody
NUF2 antibody
NUF2 NDC80 kinetochore complex component antibody
NUF2 NDC80 kinetochore complex component homolog antibody
NUF2, S. cerevisiae, homolog of antibody
NUF2_HUMAN antibody
NUF2R antibody
数据库入口号Swiss-Prot#:Q9BZD4
Uniprot
Q9BZD4
计算分子量54 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
FC: 1:50-1:100
Western blot analysis of Nuf2 on different cell lysates using anti-Nuf2 at 1/1,000 dilution.
Positive control��
Line 1: K562
Line 2: PC-3M
Line 3: THP-1
Flow cytometric analysis of A431 cells with Nuf2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
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et al,Uncovering novel functions of NUF2 in glioblastoma and MRI-based expression prediction
, (2025),
PMID:
40890233
Yes
