产品详情
产品名称CD14 Rabbit Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human;Mouse;Rat
免疫原描述Recombinant fusion protein of human CD14 (NP_001167576.1).
标记Unconjugated
别名CD14
数据库入口号Swiss Prot:P08571
GeneID:929
Uniprot
P08571
计算分子量40kDa
实际分子量70kDa
配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CD14 at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human spleen using CD14 at dilution of 1:100 (40x lens).
Immunofluorescence analysis of RAW264.7 cells using CD14 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of THP-1 cells using CD14 at dilution of 1:100. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein.
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et al,Single-cell analysis reveals the immune heterogeneity and interactions in lungs undergoing hepatic ischemia-reperfusionInInt ImmunopharmacolOn2023 NovbyChen Zhang1,Mingwei Sheng2, et al..PMID:37844464
, (2023),
PMID:
37844464
Yes
