有货
All lanes: Wnt5a Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 42 kDa Observed band size: 45 kDaExposure time: 3 seconds
All lanes:Wnt5a Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 :Wnt5a Rabbit mAb knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Wnt5a using 59447 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Wnt5a antibody (59447) ICC/IF staining of Wnt5a in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59447 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
