有货
All lanes: Atg16L1 Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : 293 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 68 kDa Observed band size: 68 kDaExposure time: 5 seconds
All lanes: Atg16L1 Rabbit mAb at 1/1k dilutionLane 1 : Mouse brain lysates Lane 2 : Rat heart lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 68 kDa Observed band size: 68 kDaExposure time: 9 seconds
All lanes:Atg16L1 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type 293T cell lysateLane 2 :Atg16L1 Rabbit mAb knockdown 293T cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human prostate tissue stained for Atg16L1 using 59470 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Atg16L1 antibody (59470) ICC/IF staining of Atg16L1 in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59470 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
