有货
All lanes: Bag3 Rabbit mAb at 1/1k dilutionLane 1 : K562 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 62 kDa Observed band size: 80 kDaExposure time: 4 seconds
All lanes:Bag3 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 :Bag3 Rabbit mAb knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Bag3 using 59486 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Bag3 antibody (59486) ICC/IF staining of Bag3 in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 59486 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
