有货
All lanes: P4HB Rabbit mAb at 1/1k dilutionLane 1 : HepG2 whole cell lysatesLane 2 : 293 whole cell lysatesLane 3 : Mouse spleen lysates Lane 4 : Rat liver lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 57 kDa Observed band size: 57 kDaExposure time: 7 seconds
All lanes:P4HB Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HAP1 cell lysateLane 2 :P4HB Rabbit mAb knockdown HAP1 cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human liver tissue stained for P4HB using 60036 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence P4HB antibody (60036) ICC/IF staining of P4HB in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 60036 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
