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CD44 Rabbit mAb #60093

是否有货: 3个工作日
产品详情

产品名称CD44 Rabbit mAb

来源种属Recombinant Rabbit

克隆性Monoclonal

亚型Rabbit IgG

纯化Affinity-chromatography

应用WB IHC ICC/IF

种属反应性Human;Mouse;Rat

免疫原描述A synthesized peptide derived from human CD44

标记Unconjugated

别名LHR; CD44; CD44 antigen; CDw44; chondroitin sulfate proteoglycan 8; CSPG8; Epican; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; HSA; HUTCH1; HUTCHI; Hyaluronate receptor; MDU2; MDU3; MIC4; MUTCH1; PGP1; Phagocytic glycoprotein 1; Soluble CD44

数据库入口号P16070

Uniprot P16070

计算分子量82kDa

实际分子量82kDa

配方Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

应用详情
WB 1:1000-1:2000 IHC 1:100-1:200 ICC/IF 1:50-1:200 IP 1:20-1:50 FC 1:20-1:100
All lanes: CD44 Rabbit mAb at 1/1k dilution
Lane 1 : U-87 MG whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 5 seconds
All lanes:CD44 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 :CD44 Rabbit mAb knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human liver tissue stained for CD44 using 60093 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for CD44 using 60093 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CD44 antibody (60093)
ICC/IF staining of CD44 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 60093 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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