yes
All lanes : OXGR1 Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : A549 whole cell lysates Lane 3 : HUVEC whole cell lysatesLysates/proteins at 20 µg per lane.Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 38 kDa Observed band size: 38 kDaExposure time: 10 seconds
All lanes : OXGR1 Rabbit mAb at 1/1k dilutionLane 1 : Mouse Kidney lysates Lane 2 : Rat Spleen lysatesLysates/proteins at 20 µg per lane.Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 38 kDa Observed band size: 38 kDaExposure time: 45 seconds
All lanes : OXGR1 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysate Lane 2 : OXGR1 knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence OXGR1 antibody (60143) 60143 staining OXGR1 in the HeLa cell line by ICC/IF. Cells were fixed with 4% ParafoX-rmaldehyde permeabilized with 0.1% Triton 100. Samples were incubated with 60143 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 60143 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.
