Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SDF1a in samples. An antibody specific for SDF1a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDF1a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDF1a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDF1a bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12).SDF-1 is produced in two forms, SDF-1α/CXCL12a and SDF-1β/CXCL12b, by alternate splicing of the same gene. SDF-1β is synthesized as a 93 amino acid (aa) precursor that contains a 21 aa signal sequence and a 72 aa mature region. The mature molecule exhibits a typical three antiparallel β-strand chemokine-like fold. There are no potential N-linked glycosylation sites. N-terminal aa s 1-8 form a receptor binding site, while aas 1 and 2 (Lys-Pro) are involved in receptor activation. The C-terminus is likely associated with heparin binding. SDF-1β circulates and undergoes proteolytic processing. SDF-1β are reported to be a monomers at neutral pH and physiologic ionic strength.