Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate RAB3B in samples. An antibody specific for RAB3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRAB3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAB3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB3B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The mammalian RAB proteins show striking similarities to the S. cerevisiae YPT1 and SEC4 proteins, Ras-related GTP-binding proteins involved in the regulation of secretion.RAB3B protein shares 78% identity with RAB3A. After endocytosis, the PIGR-dIgA complex traverses several endosomal compartments and is exocytosed at the apical plasma membrane Rab3b localized to vesicular structures containing Pigr in canine kidney cells and that GTP-bound Rab3b interacted directly with the cytoplasmic domain of Pigr. Binding of dIgA to Pigr dissociated Rab3b, but a constitutively active Rab3b mutant maintained its interaction with Pigr in dIgA-treated cells. Furthermore, GTP-locked Rab3b inhibited dIgA-stimulated transcytosis.