PIM3 Antibody - SAB | Signalway Antibody

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PIM3 Antibody#37701

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产品名称PIM3 Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antigen affinity purification.

应用WB,IHC,ELISA

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of total PIM3 protein.

免疫原类型Peptide

免疫原描述Synthetic peptide corresponding to residues near the C terminal of human pim-3 oncogene

基因/蛋白名称PIM3

别名pim-3

数据库入口号Swiss-Prot#: Q86V86
NCBI Gene ID: 415116
Gene Accssion: NP_001001852

UniprotQ86V86

实际分子量36kd

浓度1.2 mg/ml

配方Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:1000-1:5000
Immunohistochemistry: 1:50-1:200

Gel: 8%SDS-PAGELysate: 40 μgLane: Mouse brain tissue lysatePrimary antibody: 37701(PIM3 Antibody) at dilution 1/1350Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilutionExposure time: 5 seconds

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 37701(PIM3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

背景

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis.

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应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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