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位置: 首页 > 磷酸化抗体 > Myosin Light Chain 2 (Phospho-Ser19) Antibody

Myosin Light Chain 2 (Phospho-Ser19) Antibody#11114

Myosin Light Chain 2 (Phospho-Ser19) Antibody
Myosin Light Chain 2 (Phospho-Ser19) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Myosin Light Chain 2 (Phospho-Ser19) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Myosin Light Chain 2 only when phosphorylated at serine 19.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 19 (A-T-S(p)-N-V) derived from Human Myosin Light Chain 2.

基因/蛋白名称Myosin Light Chain 2

修饰Phospho

别名LC20; MLC2; MRLC1; MYRL2; MLC-2C; MMIHS4

UniprotP24844

Gene ID10398

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myosin Light Chain 2 (Phospho-Ser19) Antibody #11114 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from 293 cells untreated or treated with starvation using Myosin Light Chain 2 (Phospho-Ser19) Antibody #11114.
背景

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion

Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619.
Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627
Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256

如果您使用该产品11114发表了文章,请通知我们,让我们可以引用您的文献。

et al,FOXD3 confers chemo-sensitivity in ovarian cancer through a miR-335/DAAM1/myosin II axis-dependent mechanismInJ Ovarian ResOn2023 Jan 10byShufen Wang?1,?Yan Ma? et al..PMID:?36627652, , (2023),
PMID: 36627652
et al,Gastrodin attenuates angiotensin II-induced vascular contraction and MLCK/p-MLC2 pathway activationIn Pharm BiolOn2023 DecbyZhi Guo , Xuan Yang et al..PMID:37211627, , (2023),
PMID: 37211627
et al,Tetramethylpyrazine Suppresses the Enhanced Ca2+ Sensitivity through Inhibiting the Expression of RhoA-ROCK in Artery of Simulated Weightlessness Rats., , (2022),
PMID:
et al, Phosphorylated myosin light chain 2 (p-MLC2) as a molecular marker of antemortem coronary artery spasm.In Med Sci Monit on 2016 Sep 19 by Liliang Li, Yuhua Li et al..PMID: 27643564, , (2016),
PMID: 27643564
et al,TLR-mediated secretion of endoplasmic reticulum aminopeptidase 1 from macrophages.In J Immunol on 2014 May 1 by Yoshikuni Goto, Kenji Ogawa et al..PMID:24688025, , (2014),
PMID: 24688025
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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