产品详情
产品名称c-Src Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC IF ELISA
种属反应性Hu Ms Rt
特异性c-Src Polyclonal Antibody detects endogenous levels of c-Src protein.
免疫原描述Synthesized peptide derived from human c-Src around the non-phosphorylation site of Y419.
基因/蛋白名称c-Src
别名SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
数据库入口号Swiss-Prot: P12931
NCBI Gene ID: 6714
UniprotP12931
实际分子量60kd
浓度1mg/ml
配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
保存Store at -20˚C/1 year
应用详情
Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
Immunofluorescence: 1/200 - 1/1000.
ELISA: 1/40000.
Not yet tested in other applications.
Western Blot analysis of 293 cells using c-Src Polyclonal Antibody
Western Blot analysis of various cells using c-Src Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,c-Src Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-lung tissue. 1,c-Src Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1,c-Src Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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