产品详情
产品名称DHX58 Antibody
来源种属Mouse
克隆性Monoclonal
克隆号A3-A6
纯化ProA affinity purified
应用WB, FC
种属反应性Hu
免疫原描述Recombinant protein
别名D11LGP2 antibody
D11lgp2e antibody
DEXH (Asp Glu X His) box polypeptide 58 antibody
DEXH box polypeptide 58 antibody
DHX 58 antibody
DHX58 antibody
DHX58_HUMAN antibody
LGP 2 antibody
LGP2 antibody
Ortholog of mouse D11lgp2 antibody
Probable ATP dependent helicase LGP2 antibody
Probable ATP dependent RNA helicase DHX58 antibody
Probable ATP-dependent helicase LGP2 antibody
Probable ATP-dependent RNA helicase DHX58 antibody
Protein D11Lgp2 homolog antibody
RIG-I-like receptor LGP2 antibody
RLR antibody
RNA helicase LGP2 antibody
数据库入口号Swiss-Prot#:Q96C10
计算分子量77 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
FC: 1:50-1:200
Western blot analysis of DHX58 on human DHX58 recombinant protein using anti-DHX58 antibody at 1/1,000 dilution.
Western blot analysis of DHX58 on HEK293 (1) and DHX58-hIgGFc transfected HEK293 (2) cell lysate using anti-DHX58 antibody at 1/1,000 dilution.
Flow cytometric analysis of Hela cells with DHX58 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
背景
Helicases are enzymes that catalyze the separation of double stranded DNA or RNA by utilizing ATP. LGP2, also known as probable ATP-dependent RNA helicase DHX58, is a 678 amino acid protein belonging to the helicase family. LGP2 acts as a negative regulator of host innate immune defense against viruses by binding dsRNA produced during viral replication. The repressor domain of LGP2 binds to RIG-I, a signaling protein involved in host defenses against hepatitis C virus (HCV). By preventing RIG-I multimerization, LGP2 negatively regulates RIG-I-mediated signaling. Localized to the cytoplasm, LGP2 contains one helicase ATP-binding domain and one helicase C-terminal domain.
背景文献
1. Childs KS ?et al.?LGP2 plays a critical role in sensitizing mda-5 to activation by double-stranded RNA.PLoS One?8:e64202 (2013). 2. Childs K ?et al.?Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction.?J Virol?86:3411-21 (2012).
Yes
