产品详情
产品名称JARID2 Antibody
来源种属Mouse
克隆性Monoclonal
纯化ProA affinity purified
应用WB, ICC, FC
种属反应性Hu
免疫原描述Recombinant protein
别名JARD2 antibody
JARD2_HUMAN antibody
JARID2 antibody
JMJ antibody
Jumonji AT rich interactive domain 2 antibody
Jumonji homolog antibody
Jumonji like protein antibody
Jumonji protein antibody
Jumonji/ARID domain containing protein 2 antibody
Jumonji/ARID domain-containing protein 2 antibody
Protein Jumonji antibody
数据库入口号Swiss-Prot#:Q92833
计算分子量139 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
ICC: 1:50-1:200
FC: 1:100-1:200
Western blot analysis of JARID2 on human JARID2 recombinant protein using anti-JARID2 antibody at 1/1,000 dilution.
Western blot analysis of JARID2 on HEK293 (1) and JARID2-hIgGFc transfected HEK293 (2) cell lysate using anti-JARID2 antibody at 1/1,000 dilution.
ICC staining JARID2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with JARID2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
背景
Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.
背景文献
1. Nguyen K ?et al.?Multiple Histone Lysine Methyltransferases Are Required for the Establishment and Maintenance of HIV-1 Latency.?MBio?8:N/A (2017). 2. Puda A et al. Frequent deletions of JARID2 in leukemic transformation of chronic myeloid malignancies.Am J Hematol 87(3):245-50 (2012).
Yes
