产品详情
产品名称DIS3L2 Antibody
来源种属Mouse
克隆性Monoclonal
克隆号B2-C7
纯化ProA affinity purified
应用WB, IHC, FC
种属反应性Hu
免疫原描述Recombinant protein
别名DI3L2_HUMAN antibody
DIS3 L2 antibody
DIS3 mitotic control homolog (S. cerevisiae) like 2 antibody
DIS3 mitotic control homolog like 2 antibody
DIS3-like exonuclease 2 antibody
Dis3l2 antibody
FLJ36974 antibody
MGC42174 antibody
数据库入口号Swiss-Prot#:Q8IYB7
计算分子量100 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
FC: 1:100-1:200
Western blot analysis of DIS3L2 on human DIS3L2 recombinant protein using anti-DIS3L2 antibody at 1/1,000 dilution.
Western blot analysis of DIS3L2 on HEK293 (1) and DIS3L2-hIgGFc transfected HEK293 (2) cell lysate using anti-DIS3L2 antibody at 1/1,000 dilution.
Western blot analysis of DIS3L2 on Hela (1) and HepG2 (2) cell lysate using anti-DIS3L2 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-DIS3L2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-DIS3L2 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Jurkat cells with DIS3L2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
背景
The exosome is a multisubunit complex composed of several highly conserved subunits, some of which are 3' to 5' exoribonucleases. The complex is involved in a variety of cellular processes and is responsible for degrading unstable mRNAs that contain AU-rich (ARE) elements in their untranslated 3' region. DIS3L2 (DIS3-like exonuclease 2) is an 885 amino acid protein that is thought to function as an exonuclease and may be required for the 3' processing of pre-mRNA into mature mRNA. Defects or chromosomal translocations involving the gene encoding DIS3L2 may be associated with Marfanoid habitus, a genetic disorder characterized by abnormalities in the skeleton, eyes and cardiovascular system. DIS3L2 is expressed as five isoforms due to alternative splicing events.
背景文献
1. Du Toit A. Cytoskeleton: Formins induce nuclear actin assembly. Nat Rev Mol Cell Biol 14(6):328 (2013).
2. Astuti D et al. Germline mutations in DIS3L2 cause the Perlman syndrome of overgrowth and Wilms tumor susceptibility. Nat Genet 44(3):277-84 (2012).
Yes
