产品详情
产品名称PDK1 Rabbit mAb
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
克隆号JA67-30
纯化ProA affinity purified
应用WB, IHC, IP, FC
种属反应性Hu, Ms, Rt
免疫原描述recombinant protein
数据库入口号Swiss-Prot#:Q15118
计算分子量49 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC:1:50-1:200
FC: 1:50-1:100
Western blot analysis of PDK1 on rat heart tissue lysate using anti-PDK1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDK1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PDK1 antibody. Counter stained with hematoxylin.
ICC staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PDK1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of NIH-3T3 cells with PDK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
背景
Mitochondrial pyruvate dehydrogenase (PDH) catalyzes the oxidative decarboxylation of pyruvate and plays a central role in the regulation of homeostasis of carbohydrate fuels in mammals. PDH activity is controlled by a phosphorylation/dephosphorylation cycle, phosphorylation leading to inactivation and dephosphorylation leading to reactivation of PDH. The phosphorylation of PDH is catalyzed by pyruvate dehydrogenase kinase (PDK), the activity of which is stimulated by the products of PDH catalysis. PDK1 consists of alpha and beta subunits; the kinase activity resides in the alpha subunit. Three PDK isoenzymes have been identified in humans (PDK1, 2 and 3) and two have been identified in rodent (PDK1 and 2).
背景文献
1. Du L ?et al.?Overexpression of PIK3CA in murine head and neck epithelium drives tumor invasion and metastasis through PDK1 and enhanced TGF? signaling.?Oncogene?35:4641-52 (2016).
2. Lelliott CJ et al. Monoclonal antibody targeting of fibroblast growth factor receptor 1c ameliorates obesity and glucose intolerance via central mechanisms. PLoS One 9:e112109 (2014).
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