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Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using #43042 at dilution 1/20.
Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using #43042 at dilution 1/20.
Western blot analysis of MRPS30 expression in Hela (A), HepG2 (B) whole cell lysates.
Immunofluorescent analysis of MRPS30 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
