产品详情
产品名称XAB2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification
应用IHC WB
种属反应性Hu Ms Rt
特异性The antibody detects endogenous levels of total XAB2 protein.
免疫原类型peptide
免疫原描述Synthetic peptide of human XAB2
基因/蛋白名称XAB2
别名HCNP; HCRN; SYF1; NTC90
数据库入口号Swiss-Prot#: Q9HCS7
NCBI Gene ID: 56949
UniprotQ9HCS7
计算分子量100kd
浓度0.7mg/ml
配方Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:200-1000
Immunohistochemistry: 1: 20-100
Gel: 6%SDS-PAGE
Lysate: 40 μg, Lane 1-6: A431,K562,Hela,HEPG2,PC3 and 293T cell lysates,
Primary antibody:XAB2 antibody at dilution 1/250,
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
Exposure time: 1 second
The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using XAB2 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using XAB2 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
背景
HCNP, also known as XAB2 (Xeroderma pigmentosum group A (XPA) binding protein 2), HCRN, SYF1 or NTC90, is a nuclear protein that participates in transcription, transcription-coupled repair (TCR) and pre-mRNA splicing. It contains fifteen tetratricopeptide repeat motifs and associates with nucleotide excision repair machinery. More specifically, HCNP associates with Cockayne syndrome group A and B proteins (CSA and CSB), RNA Polymerase II (Pol II) and XPA in response to DNA damage and is believed to function in the TCR pathway. HCNP also functions as an essential component of a pre-mRNA splicing complex of the spliceosome (composed of AQR (aquarius), PRP19, CCDC16, HCNP, ISY1 and Cyclophilin E) and is required for proper RNA synthesis in the cell. In addition, HCNP functions as a component of the RAR corepressor complex with RAR? and HDAC3 and exhibits an inhibitory effect on ATRA-induced cell differentiation. This suggests that HCNP may function as useful target in cancer therapy.
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