产品详情
产品名称LC3B Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IHC,IF
种属反应性Human,Mouse,Rat
免疫原描述A synthetic peptide of human LC3B
别名MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta
数据库入口号Swiss-Prot#:Q9GZQ8
NCBI Gene ID:81631
计算分子量14kDa, 16kDa
配方Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using LC3B antibody at 1:1000 dilution. C6 cells were treated by Chloroquine (50 ��M) for 20 hours. Hela cells were treated by Chloroquine (50 ��M) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 ��M) for 20 hours.
Western blot analysis of extracts of pig liver, using LC3B antibody.
Immunohistochemistry of paraffin-embedded rat heart using LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using LC3B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using LC3B antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of rat brain using LC3B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
背景
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.