Yes
Western blot analysis of GPR37L1 expression in SKOVCAR3 (A), HEK293T (B), A549 (C), LO2 (D), EC9706 (E) whole cell lysates
Immunofluorescent analysis of GPR37L1 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
