免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > ELISA试剂盒 > Mouse IL-1a ELISA kit

Mouse IL-1a ELISA kit#EK0505

Mouse IL-1a ELISA kit
是否有货: 1-2周
产品总价:
产品详情

产品名称Mouse IL-1a ELISA kit

简述ELISA Kit

应用ELISA

种属反应性Ms

特异性Natural and recombinant Mouse IL-1a Ligand

交叉反应性No significant interference observed with available related molecules.

基因/蛋白名称Mouse IL-1a

应用详情
Detect Range: 1.8 - 125 pg/mL
Sensitivity: 1pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hour
Detection method: Colorimetric
Representative standard curve for IL-1α ELISA. IL-1α was diluted in serial two-fold steps in Sample Diluent.
产品描述
  • Aluminium pouches with a Microwell Plate coated with antibody to mouse IL-1α (8X12)
  • 2 vials mouse IL-1α Standard lyophilized, 250 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-mouse IL-1α antibody
  • 2 vials Streptavidin-HRP solution,
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
背景

Interleukin-1 alpha(IL-1a) is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. IL-1 alpha is synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17 kDa. The IL-1a precursor reportedly shows full biological activity in the EL-4 assay. Circulating levels of IL-1a are normally low and only rise after stimulation by agents such as those produced byinflammation, infection or microbial endotoxins. IL-1a possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.

背景文献

Nicklin, M.J. et al.(1994) Genomics 19:382.

March, C.J. et al. (1985) Nature. 315:641.

Bankers-Fulbright, J.L et al(1996) Life Sci. 59:61.

Watanabe, N. and Kobayashi, Y. (1994) Cytokine 6:597.

Hauser, C. et al. (1986) 136:3317.

Gahring, L.C. et al. (1985) 76:1585.

Hauser, C. et al. (1986). J.Immunol.136:3317.

Barland, C.O. et al.(2004). J. Invest.Dermatol. 122:330.

Hu, B. et al. (2003). Proc. Natl. Acad. Sci. U.S.A. 100:10008.

Arend, W.P. et al (1998) Annu. Rev. Immunol. 16:27

如果您使用该产品EK0505发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com