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Immunofluorescence analysis of human-stomach tissue. 1,CD23 Monoclonal Antibody(1E9)(red) was diluted at 1:200(4˚C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1,CD23 Monoclonal Antibody(1E9)(red) was diluted at 1:200(4˚C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,CD23 Monoclonal Antibody(1E9) was diluted at 1:200(4˚C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98˚C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CD23 Monoclonal Antibody(1E9) was diluted at 1:200(4˚C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98˚C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,CD23 Monoclonal Antibody(1E9) was diluted at 1:200(4˚C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98˚C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.
