产品详情
产品名称PI3 Kinase p85/p55 (phospho-Tyr467/199)Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB,IF,IHC
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of total PI3 Kinase p85/p55 only when phosphorylated at tyrosine 467/199.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85/p55.
基因/蛋白名称PI3 Kinase p85/p55
修饰Phospho
别名p85, AGM7, p85-ALPHA, p55, p55-GAMMA,PIK3R1
数据库入口号Swiss-Prot: P27986 Q92569
NCBI Protein: NP_001229395.1 NP_001107644.1
实际分子量55,85kd
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 55, 85kd
Western blotting: 1:500~1:1000
IHC: 1/100 - 1/300
IF 1:100-1:500
背景
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1./Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Stoyanov, B. et al. (1995) Science 269, 690-3.
Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
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