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位置: 首页 > 磷酸化抗体 > Vimentin (Phospho-Ser56) Antibody

Vimentin (Phospho-Ser56) Antibody#11940

Vimentin (Phospho-Ser56) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Vimentin (Phospho-Ser56) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of Vimentin only when phosphorylated at serine 56.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 56 (A-S-S(p)-P-G) derived from Human Vimentin.

基因/蛋白名称Vimentin

修饰Phospho

别名VIME; vimentin;

数据库入口号Swiss-Prot#: P08670;
NCBI Gene#: 7431;
NCBI Protein#: NP_003371.2

UniprotP08670

实际分子量54kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情
Western blotting: 1:500~1:1000
Western blot analysis of extracts from A549 cells treated with Nocodazole using Phospho-Vimentin (Ser56) antibody #11940.The lane on the right is treated with the antigen-specific peptide.
背景

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

Matsuyama M, et al. (2013)J Biol Chem 288, 35626-35. Haolong C, et al. (2013)PLoS One 8, e73900. Kotula E, et al. (2013)PLoS One 8, e80313.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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