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位置: 首页 > 磷酸化抗体 > BRCA2 (Phospho-Ser3291) Antibody

BRCA2 (Phospho-Ser3291) Antibody#11840

BRCA2 (Phospho-Ser3291) Antibody
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产品总价:
产品详情

产品名称BRCA2 (Phospho-Ser3291) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of BRCA2 only when phosphorylated at serine 3291.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 3291 (F-V-S(p)-P-A) derived from Human BRCA2.

基因/蛋白名称BRCA2

修饰Phospho

别名 BRCC2; BRCA2; subunit 2; BRCA1/BRCA2-containing complex;

数据库入口号Swiss-Prot#: P51587;
NCBI Gene#: 675;
NCBI Protein#: NP_000050.2.

UniprotP51587

实际分子量>350kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from 293 cells using BRCA2 (Phospho-Ser3291) Antibody #11840.The lane on the right is treated with the antigen-specific peptide.
背景

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.

Wooster R., Nature 378:789-792(1995).
Tavtigian S.V., Nat. Genet. 12:333-337(1996).
Dunham A., Nature 428:522-528(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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