GAPDH Antibody - SAB | Signalway Antibody

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GAPDH Antibody#21612

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产品名称GAPDH Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total GAPDH protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.252~256(P-A-K-Y-D) derived from Human GAPDH.

基因/蛋白名称GAPDH

别名G3PD; GAPD; MGC88685

数据库入口号Swiss-Prot: P04406
NCBI Protein: NP_002037.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 37kd
Western blotting: 1:5000~1:10000

Western blot analysis of extract from Hela, 293 and 3T3 cells using GAPDH Antibody #21612

Western blot analysis of extracts of various cell lines, using GAPDH antibody at 1:20000 dilution.

背景

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Ercolani L., Florence B., Denaro M., Alexander M. J. Biol. Chem. 263:15335-15341(1988)
Tisdale E.J.J. Biol. Chem. 277:3334-3341(2002)

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操作手册

注释

应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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GAPDH Antibody#21612

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GAPDH Antibody#21612

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种属反应性

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