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IKK- alpha/ beta (Phospho-Ser176/177) Antibody#11931

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产品详情

产品名称IKK- alpha/ beta (Phospho-Ser176/177) Antibody

来源种属Rabbit

克隆性Polyclonal

亚型IgG

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB,IHC,IF,ELISA

种属反应性Human;Mouse;Rat

特异性The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK-alpha/beta.

基因/蛋白名称IKK- alpha/ beta

标记Unconjugated

别名FLJ40509; I-kappa-B kinase; IKBKB; kinase beta; NFKBIKB

数据库入口号Swiss-Prot#: O15111/O14920;
NCBI Gene#: 1147;
NCBI Protein#: NP_001269.3

Uniprot O15111/O14920

计算分子量85 kDa

实际分子量85 kDa

浓度1.0mg/ml

配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

保存Store at -20˚C/1 year

应用详情
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200
Immunohistochemistry analysis of paraffin-embedded human heart, using #11931. The picture on the right is blocked with the phospho peptide.
All lanes : IKK- alpha/ beta (Phospho-Ser176/177) Antibody at 1/500 dilution
Lane 1 : Jurkat treated with 20ng/ml TNFa for 15 minutes whole cell lysates
Lane 2: HUVEC treated with 100ng/ml EGF for 30 minutes whole Cell lysate
Lane 3: Hela treated with 100ng/ml EGF for 30 minutes whole Cell lysate
Lane 4: HepG2 treated with 100ng/ml EGF for 30 minutes whole Cell lysate
Lane 5 : Mouse brain lysate
Lane 6 : Mouse lung lysate
Lane 7 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 5 seconds
All lanes : IKK- alpha/ beta (Phospho-Ser176/177) Antibody at 1/500 dilution
Lane 1 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa
Exposure time: 7 seconds

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.

Chandrakesan P, et al. (2010)J Biol Chem 285, 33485-98
Hinz M, et al. (2010£© Mol Cell 40, 63-74
Choudhary S, Lu M, Cui R, Brasier AR (2007)Mol Endocrinol 21, 2203-17

如果您使用该产品11931发表了文章，请通知我们，让我们可以引用您的文献。

et al,Inhibition of BET Protein Function Suppressed the Overactivation of the Canonical NF-κB Signaling Pathway in 6-OHDA-Lesioned Rat Model of Levodopa-Induced Dyskinesia , (2022),
PMID: 35801173
et al,Anti-inflammatory activity and mechanism of action of Banxia Xiexin Decoction on DSS-induced ulcerative colitis in mice , (2025),
PMID: 41161626

操作手册

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

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