Yes
All lanes : STAT3 (Phospho-Ser727) Rabbit mAb at 1/1k dilutionLane 1 :A431 cell lysate Lane 2 :A431 cell lysate treated with 100ng/ml EGF 10minlysate/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 88 kDa Observed band size: 98 kDaExposure time: 5 seconds
Formalin-fixed;paraffin-embedded rat brain tissue stained for STAT3 (Phospho-Ser727) using 14152 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded rat kidney tissue stained for STAT3 (Phospho-Ser727) using 14152 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed;paraffin-embedded human brain tissue stained for STAT3 (Phospho-Ser727) using 14152 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence STAT3 (Phospho-Ser727) antibody (14152)
ICC/IF staining of STAT3 (Phospho-Ser727) in A431 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14152 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
