产品详情
产品名称GAPDH antibody
来源种属Rabbit
克隆性Polyclonal
纯化Purified by antigen-affinity chromatography.
应用WB IHC IF
种属反应性Human
免疫原类型Peptide
免疫原描述Synthetic peptide contain a sequence corresponding to a region within amino acids 84 and 145 of GAPDH
基因/蛋白名称GAPDH
标记Unconjugated
数据库入口号NCBI Gene ID: 2597
NCBI mRNA#: NM_002046
NCBI Protein#: NP_002037
浓度1mg/ml
配方Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.
应用详情
Predicted MW: 36kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
GAPDH antibody diluted at 1: 1000
Immunohistochemical analysis of paraffin-embedded Gastric CA, using GAPDH antibody at 1: 100 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using GAPDH antibody at 1: 100 dilution.
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
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et al,Inhibiting cancer metastasis with water-solubilized membrane receptor CXCR4QTY-Fc as a molecular trap
, (2025),
PMID:
40845831