产品详情
产品名称CS Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were purified by affinity purification using immunogen.
应用WB,IF
种属反应性Human;Mouse;Rat
特异性The antibody detects endogenous level of total CS protein.
免疫原类型Recombinant Protein
免疫原描述Recombinant protein of human CS.
基因/蛋白名称CS
标记Unconjugated
数据库入口号Swiss-Prot:O75390
NCBI Gene ID:1431
Uniprot
O75390
实际分子量51KD
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
WB 1:500 - 1:2000
IF 1:50 - 1:200
Immunofluorescence analysis of MCF-7 cells using CS antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CS antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 15s.
Confocal immunofluorescence analysis of U2OS cells using CS Polyclonal antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found.
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et al,Saikosaponin D ameliorates sepsis-induced acute lung injury by maintaining alveolar epithelial barrier integrity and inhibiting ferroptosis via Nrf2/HO-1 pathway
, (2025),
PMID:
40569790
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