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ACTIN Antibody#35537

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产品详情

产品名称ACTIN Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antigen affinity purification.

应用WB,ELISA

种属反应性Plant

特异性The antibody detects endogenous levels of total ACTIN protein.

免疫原类型Peptide

免疫原描述Synthetic peptide corresponding to residues near the N terminal of Arabidopsis thaliana actin

基因/蛋白名称ACTIN

标记Unconjugated

别名ACTB

数据库入口号Swiss-Prot#: P53497
NCBI Gene ID: 823805
Gene Accssion: NP_190236.1

Uniprot P53497

实际分子量42kd

浓度0.8 mg/ml

配方Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500-1:2000

Gel: 8%SDS-PAGE;
Lysate: 40 ug;
Lane: Arabidopsis protein lysates;
Primary antibody: ACTIN Antibody at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds;

Member of actin subclass composed of ACT12 and ACT4. RNA is expressed at very low levels in vegetative organs, low levels in flowers and very high levels in pollen. Expression of an ACT12/GUS fusion was found in vascular tissues, tapetum, developing and mature pollen, the root cap and in a ring of pericycle tissues during lateral root initiation and early development.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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