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Cyclin D1 Rabbit mAb#48619

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产品详情 图片 产品描述 发表文献(1) 其它
产品详情

产品名称Cyclin D1 Rabbit mAb

克隆号SA38-08

来源种属Recombinant Rabbit

克隆性Monoclonal antibody

纯化ProA affinity purified

应用WB, ICC/IF, IHC, IP

种属反应性Human;Mouse;Rat

免疫原描述recombinant protein

标记Unconjugated

别名AI327039 antibody
B cell CLL/lymphoma 1 antibody
B cell leukemia 1 antibody
B cell lymphoma 1 protein antibody
B-cell lymphoma 1 protein antibody
BCL 1 antibody
BCL-1 antibody
BCL-1 oncogene antibody
BCL1 antibody
BCL1 oncogene antibody
ccnd1 antibody
CCND1/FSTL3 fusion gene, included antibody
CCND1/IGHG1 fusion gene, included antibody
CCND1/IGLC1 fusion gene, included antibody
CCND1/PTH fusion gene, included antibody
CCND1_HUMAN antibody
cD1 antibody
Cyl 1 antibody
D11S287E antibody
G1/S specific cyclin D1 antibody
G1/S-specific cyclin-D1 antibody
Parathyroid adenomatosis 1 antibody
PRAD1 antibody
PRAD1 oncogene antibody
U21B31 antibody

数据库入口号Swiss-Prot#:P24385

Uniprot P24385

计算分子量34 kDa

实际分子量36 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
ICC staining Cyclin D1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cyclin D1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes: Cyclin D1 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : PC12 whole cell lysates
Lane 3 : SH-SY5Y whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 36 kDa
Exposure time: 4 seconds
All lanes: Cyclin D1 Rabbit mAb at 1/1k dilution
Lane 1 : Rat kidney lysates
Lane 2 : Rat lung lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse lung lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 36 kDa
Exposure time: 8 seconds
All lanes: Cyclin D1 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : Cyclin D1 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.

如果您使用该产品48619发表了文章，请通知我们，让我们可以引用您的文献。

et al,Long non-coding RNA TRIM52-AS1 sponges microRNA-577 to facilitate diffuse large B cell lymphoma progression via increasing TRIM52 expression. In Hum Cell on 2022 Jul by Fang Zhao, Shucheng Li,et al..PMID:35676608 , (2022),
PMID: 35676608

操作手册

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu人
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk猴
  • Rb兔
  • B牛
  • D狗
  • P猪
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk鸡
;

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