产品详情
产品名称Hes1 Rabbit mAb
克隆号SC06-21
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名bHLHb39 antibody C-HAIRY1 antibody c-hairy1A antibody Class B basic helix-loop-helix protein 39 antibody FLJ20408 antibody Hairy and enhancer of split 1 (Drosophila) antibody Hairy and enhancer of split 1 antibody Hairy homolog (Drosophila) antibody Hairy homolog antibody Hairy like antibody Hairy, Drosophila, homolog of antibody Hairy-like protein antibody Hairy/enhancer of split, Drosophila, homolog of, 1 antibody HAIRY1 antibody HES-1 antibody hes1 antibody Hes1 hairy and enhancer of split 1 (Drosophila) antibody HES1_HUMAN antibody HHL antibody HL antibody HRY antibody MGC129109 antibody OTTHUMP00000209031 antibody RHL antibody Transcription factor HES-1 antibody
数据库入口号Swiss-Prot#:Q14469
Uniprot
Q14469
计算分子量30 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of Hes1 on different lysates using anti-Hes1 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SH-SY-5Y
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. Counter stained with hematoxylin.
ICC staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Hes1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of sh-sy-5y cells with Hes1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The Drosophila Hairy and enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homolog of Drosophila Hairy and enhancer of split are the HES gene family members HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homolog of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues.
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et al,Unraveling the Oncogenic Characteristics of the Cytolinker, Plectin, in Esophageal Squamous Cell Carcinoma
, (2025),
PMID:
40449847
Yes
